Protein-protein and protein ligand interactions
Understanding how proteins interact with one another, and with other ligands in the cell, is a central theme in biology.
Our group uses a wide range of biochemical and biophysical methods to investigate such interactions. Shown in the figure above, from left to right are:
- the &alpha-Hb binding face (red spheres) of &alpha-Hb binding protein (AHSP), mapped using NMR methods,
- the location of residues in AHSP that are critical (red) or important (orange) for high binding affinity determined from mutagenesis,
- the binding site for hemin (red/yellow) in human heme-binding protein 22 kDa (hbp-22).
Other methods used in the group include analytical ultracentrifugation, light scattering (dynamic and static), and isothermal titration calorimetry.
- Dr David Gell (Senior Research Fellow)